Assuming Shakespeare/literature film quotes don't count...
My Top 5:
1. Anything Groucho Marx ever said in any of the Marx Bros. Films...ever.
2. "You're all individuals!"
"YES! WE'RE ALL INDIVIDUALS!"
"You're all different!"
"YES! WE'RE ALL DIFFERENT!"
("I'm not.")
(And if you don't know what movie that's from...stone him for saying Jehov--aaaaaahhh!)
3. "On the page it looked nothing. The beginning simple, almost comic. Just a pulse - bassoons and basset horns - like a rusty squeezebox. Then suddenly - high above it - an oboe, a single note, hanging there unwavering, till a clarinet took over and sweetened it into a phrase of such delight! This was no composition by a performing monkey! This was a music I'd never heard. Filled with such longing, such unfulfillable longing, it had me trembling. It seemed to me that I was hearing the very voice of God."
--Salieri describing Mozart's music from "Amadeus"
4. "here's a passage I got memorized. Ezekiel 25:17. "The path of the righteous man is beset on all sides by the inequities of the selfish and the tyranny of evil men. Blessed is he who, in the name of charity and good will, shepherds the weak through the valley of the darkness, for he is truly his brother's keeper and the finder of lost children. And I will strike down upon thee with great vengeance and furious anger those who attempt to poison and destroy My brothers. And you will know I am the Lord when I lay My vengeance upon you." Now... I been sayin' that shit for years. And if you ever heard it, that meant your ass. You'd be dead right now. I never gave much thought to what it meant. I just thought it was a cold-blooded thing to say to a motherfucker before I popped a cap in his ass. But I saw some shit this mornin' made me think twice. See, now I'm thinking: maybe it means you're the evil man. And I'm the righteous man. And Mr. 9mm here... he's the shepherd protecting my righteous ass in the valley of darkness. Or it could mean you're the righteous man and I'm the shepherd and it's the world that's evil and selfish. And I'd like that. But that shit ain't the truth. The truth is you're the weak. And I'm the tyranny of evil men. But I'm tryin', Ringo. I'm tryin' real hard to be the shepherd."
--Jules, from "Pulp Fiction" and come on...you HAVE to know that one!
And finally...sorry, it has to be a tie for the Star Trek Nerd Spot...
5. Tie between:
"He tasks me--he tasks me and I shall have him. I'll chase him round the moons of Nibia and round the Antares maelstrom and round perdition's flames before I give him up!"
--Khan, playing with Ahab's quote from "Moby Dick," in "Star Trek II: The Wrath of Khan"
AND...
"They invade our space, and we fall back. They assimilate entire worlds, and we fall back. Not again! The line must be drawn HERE! THIS FAR and *NO FURTHER!* And *I* will make them *PAY* for what they've done!"
--Picard, "Star Trek: First Contact"